University Hospital of Wurzburg 8 articles published in JoVE Cancer Research Cultivating Ex Vivo Patient-Derived Glioma Organoids Using a Tissue Chopper Marah Alsalkini1, Veronika Cibulková1, Maria Breun2, Almuth F. Kessler2, Tim Schulz2, Andrea Cattaneo2, Christoph Wipplinger2, Julian Hübner3, Ralf-Ingo Ernestus2, Thomas Nerreter3, Camelia M. Monoranu4, Carsten Hagemann1, Mario Löhr2, Vera Nickl2 1Section Experimental Neurosurgery, Department of Neurosurgery, University Hospital Würzburg, 2Department of Neurosurgery, University Hospital Würzburg, 3Department of Hematology, University Hospital Würzburg, 4Department of Neuropathology, Institute of Pathology, University Hospital Würzburg This study introduces an automated method to generate patient-derived glioblastoma 3-dimensional organoids utilizing a tissue chopper. The method provides a suitable and effective approach to obtain such organoids for therapeutical testing. Medicine Guided Endodontics: Three-Dimensional Planning and Template-Aided Preparation of Endodontic Access Cavities Wadim Leontiev1, Thomas Connert1, Roland Weiger1, Dorothea Dagassan-Berndt2, Gabriel Krastl3, Eva Magni1 1Department of Periodontology, Endodontology and Cariology, University Center for Dental Medicine Basel UZB, University of Basel, 2Center for Dental Imaging, University Center for Dental Medicine, University of Basel, 3Department of Conservative Dentistry and Periodontology, University Hospital of Würzburg Guided endodontics describes a template-aided approach for access cavity preparation. The procedure requires cone-beam computed tomography and a surface scan to produce a template. An incorporated sleeve guides the drill to the target point. This allows the preparation of minimally invasive endodontic access cavities in calcified teeth. Medicine Dynamic Navigation in Endodontics: Guided Access Cavity Preparation by Means of a Miniaturized Navigation System Wadim Leontiev1, Thomas Connert1, Roland Weiger1, Gabriel Krastl2, Eva Magni1 1Department of Periodontology, Endodontology and Cariology, University Center for Dental Medicine Basel UZB, University of Basel, 2Department of Conservative Dentistry and Periodontology, University Hospital of Würzburg Dynamic navigation systems (DNS) provide real-time visualization and guidance to the operator during endodontic access cavities preparation. The planning of the procedure requires three-dimensional imaging utilizing cone beam computed tomography and surface scans. After the export of the planning data to the DNS, access cavities can be prepared with minimal invasion. Neuroscience Multifactorial Assessment of Motor Behavior in Rats after Unilateral Sciatic Nerve Crush Injury Susanne Knorr1, Lisa Rauschenberger1, Tami Lang1, Jens Volkmann1, Chi Wang Ip1 1Department of Neurology, University Hospital of Würzburg We provide a protocol for the assessment of motor behavior via a behavioral test battery in rats after sciatic nerve crush injury. Neuroscience Implantation of Osmotic Pumps and Induction of Stress to Establish a Symptomatic, Pharmacological Mouse Model for DYT/PARK-ATP1A3 Dystonia Lisa Rauschenberger1, Susanne Knorr1, Jens Volkmann1, Chi Wang Ip1 1Department of Neurology, University Hospital of Würzburg We provide a protocol to generate a pharmacological DYT/PARK-ATP1A3 dystonia mouse model via implantation of cannulas into basal ganglia and cerebellum connected to osmotic pumps. We describe the induction of dystonia-like movements via application of a motor challenge and the characterization of the phenotype via behavioral scoring systems. Bioengineering Site-Directed Immobilization of Bone Morphogenetic Protein 2 to Solid Surfaces by Click Chemistry Claudia Siverino1, Barbara Tabisz2, Tessa Lühmann3, Lorenz Meinel3, Thomas Müller4, Heike Walles1,2, Joachim Nickel1,2 1Fraunhofer-Institut für Grenzflächen- und Bioverfahrenstechnik (IGB), Translationszentrum Würzburg 'Regenerative Therapien für Krebs- und Muskuloskelettale Erkrankung', Institutsteil Würzburg, 2Lehrstuhl für Tissue Engineering und Regenerative Medizin, Universitätsklinikum Würzburg, 3Lehrstuhl für Pharmazeutische Technologie und Biopharmazie, Universität Würzburg, 4Lehrstuhl für molekulare Pflanzenphysiologie und Biophysik, Julius-von-Sachs Institut für Biowissenschaften, Universität Würzburg Biomaterials doped with Bone Morphogenetic Protein 2 (BMP2) have been used as a new therapeutic strategy to heal non-union bone fractures. To overcome side effects resulting from an uncontrollable release of the factor, we propose a new strategy to site-directly immobilize the factor, thus creating materials with improved osteogenic capabilities. Bioengineering Generation of a Three-dimensional Full Thickness Skin Equivalent and Automated Wounding Angela Rossi*1, Antje Appelt-Menzel*1, Szymon Kurdyn1, Heike Walles1,2, Florian Groeber2 1Department for Tissue Engineering and Regenerative Medicine, University Hospital Würzburg, 2Translational Center Würzburg, Regenerative Therapies in Oncology and Musculoskelettal Disease, Würzburg Branch of the Fraunhofer-Institute Interfacial Engineering and Biotechnology, IGB The goal of this protocol is to build up a three-dimensional full thickness skin equivalent, which resembles natural skin. With a specifically constructed automated wounding device, precise and reproducible wounds can be generated under maintenance of sterility. Bioengineering Tissue Engineering of a Human 3D in vitro Tumor Test System Corinna Moll*1, Jenny Reboredo*1, Thomas Schwarz1, Antje Appelt1, Sebastian Schürlein1, Heike Walles1, Sarah Nietzer1 1Department of Tissue Engineering and Regenerative Medicine, University Hospital Würzburg Methods to create human 3D tumor tissues as test systems are described. These technologies are based on a decellularized Biological Vascularized Scaffold (BioVaSc), primary human cells and a tumor cell line, which can be cultured under static as well as under dynamic conditions in a flow bioreactor.
