Helmholtz Zentrum Munich 1 article published in JoVE Medicine Heterotopic Cervical Heart Transplantation in Mice Thomas Ratschiller1,4, Marcus-André Deutsch1, Julia Calzada-Wack2, Frauke Neff2, Christiane Roesch3, Ralf Guenzinger3, Ruediger Lange3, Markus Krane3 1Department of Cardiovascular Surgery, Division of Experimental Surgery, German Heart Center Munich, Technische Universität München, 2Institute of Pathology, Helmholtz Zentrum Munich, 3Department of Cardiovascular Surgery, Division of Experimental Surgery, German Heart Center Munich, Technische Universität München, 4Department of Cardiovascular and Thoracic Surgery, General Hospital Linz The cuff technique shortens and facilitates the model of heterotopic cervical heart transplantation in mice by avoiding technically challenging suture anastomoses of small vessels. The technical details shown in this video paper should allow researches to establish this model in their laboratories.