Institut Pasteur, Universite de Paris Cite, CNRS UMR 1 article published in JoVE Biology Capturing Cytoskeleton-Based Agitation of the Mouse Oocyte Nucleus Across Spatial Scales Gaëlle Letort1,2, Philippe Mailly1, Adel Al Jord1,3, Maria Almonacid1 1Center for Interdisciplinary Research in Biology (CIRB), Collège de France, CNRS, INSERM, Université PSL, 2Department of Developmental and Stem Cell Biology, Institut Pasteur, Université de Paris Cité, CNRS, 3Centre for Genomic Regulation (CRG), The Barcelona Institute of Science and Technology This protocol provides an experimental framework to document the physical impact of the cytoskeleton on nuclear shape and the internal membrane-less organelles in the mouse oocyte system. The framework can be adapted for use in other cell types and contexts.
