University of Athens Medical School 1 article published in JoVE Immunology and Infection Cecal Ligation and Puncture-induced Sepsis as a Model To Study Autophagy in Mice Ilias I. Siempos1,3, Hilaire C. Lam1, Yan Ding2, Mary E. Choi2, Augustine M. K. Choi1, Stefan W. Ryter1 1 Experimental sepsis can be induced in mice using the cecal ligation and puncture (CLP) method. Current protocols to assess autophagy in vivo in the context of CLP-induced sepsis are presented here: A protocol for measuring autophagy using (GFP)-LC3 mice, and a protocol for measuring autophagosome formation by electron microscopy.