IMBB FORTH 1 article published in JoVE Genetics Using the GAL4-UAS System for Functional Genetics in Anopheles gambiae Beth Crawford Poulton1, Fraser Colman1, Amalia Anthousi1,2,3, Linda Grigoraki1, Adriana Adolfi1, Amy Lynd1, Gareth John Lycett1 1Department of Vector Biology, Liverpool School of Tropical Medicine, 2IMBB FORTH, 3Department of Biology, University of Crete The bipartite GAL4-UAS system is a versatile tool for modification of gene expression in a controlled spatiotemporal manner which permits functional genetic analysis in Anopheles gambiae. The procedures described for using this system are a semi-standardized cloning strategy, sexing and screening of pupae for fluorescent protein markers and embryo fixation.