CNR Institute of Clinical Physiology 1 article published in JoVE Medicine High-Resolution Cardiac Positron Emission Tomography/Computed Tomography for Small Animals Daniele Panetta1, Maria Angela Guzzardi1, Federica La Rosa1, Federico Granziera1,2, Domiziana Terlizzi3, Claudia Kusmic1, Patricia Iozzo1 1CNR Institute of Clinical Physiology, 2Sant’Anna School of Advanced Studies, 3Fondazione Regione Toscana/CNR “G. Monasterio” Here, we present an experimental imaging protocol for the quantification of cardiac function and morphology using high-resolution positron emission tomography/computed tomography for small animals. Both mice and rats are considered, discussing the different requirements of computed tomography contrast agents for the two species.