Akademiska sjukhuset 1 article published in JoVE Immunology and Infection Efficient Isolation Protocol for B and T Lymphocytes from Human Palatine Tonsils Farzaneh Assadian1, Karl Sandström2, Göran Laurell2, Catharina Svensson1, Göran Akusjärvi1, Tanel Punga1 1Department of Medical Biochemistry and Microbiology, Uppsala Biomedical Center, Uppsala University, 2Department of Surgical Sciences, Otolaryngology and Head & Neck Surgery, Akademiska sjukhuset Palatine tonsils are a rich source of B and T lymphocytes. Here we provide an easy, efficient and rapid protocol to isolate B and T lymphocytes from human palatine tonsils. The method described has been specifically adapted for studies of the viral etiology of tonsil inflammation known as tonsillitis.