Centro Medico Nacional 20 de Noviembre 2 articles published in JoVE Medicine Predicting Amputation using Local Circulating Mononuclear Progenitor Cells in Angioplasty-treated Patients with Critical Limb Ischemia Juan Antonio Suárez-Cuenca1, Eduardo Vera-Gómez1, Alejandro Hernández-Patricio1, Atzín Suá Ruíz-Hernández1, Juan Ariel Gutiérrez-Buendía1, Carlos Ramiro Zamora-Alemán1, Alberto Melchor-López1, Yasser Alberto Rizo-García2, Oscar Antonio Lomán-Zúñiga2, Ignacio Escotto-Sánchez2, Juan Miguel Rodríguez-Trejo2, Rebeca Pérez-Cabeza de Vaca3, Mario Antonio Téllez-González3, Paul Mondragón-Terán3 1Experimental Metabolism and Clinical Research Laboratory, Clinical Research Department, Division of Biomedical Research, Centro Médico Nacional 20 de Noviembre, ISSSTE, 2Vascular Surgery and Angiology Department, Centro Médico Nacional 20 de Noviembre, ISSSTE, 3Regenerative Medicine and Tissue Engineering Laboratory; Coordination of Research, Centro Médico Nacional 20 de Noviembre, ISSSTE Lower limb amputation may occur even after angioplasty of obstructed vessels in Critical Limb Ischemia (CLI). Mononuclear Progenitor Cells (MPCs) reflect vascular repair. The present protocol describes the quantification of MPCs from circulation close to angioplasty, and its relationship with endothelial dysfunction and prediction of lower limb amputation. Medicine Coronary Progenitor Cells and Soluble Biomarkers in Cardiovascular Prognosis after Coronary Angioplasty Juan Antonio Suárez-Cuenca1, Rogelio Robledo-Nolasco2, Marco Antonio Alcántara-Meléndez2, Luis Javier Díaz-Hernandez1, Eduardo Vera-Gómez1, Alejandro Hernández-Patricio1, Karla Susana Sánchez-Díaz1, Juan Ariel Gutiérrez-Buendía1, Alejandra Contreras-Ramos3, Atzin Suá Ruíz-Hernández1, Rebeca Pérez-Cabeza de Vaca1, Paul Mondragón-Terán1 1Experimental Metabolism and Clinical Research Laboratory & Regenerative Medicine and Tissue Engineering Laboratory, 2Hemodynamics Unit, Cardiology Department, Centro Médico Nacional "20 de Noviembre" ISSSTE, 3 Development of major adverse cardiovascular events, which impact cardiovascular prognosis after coronary angioplasty, are influenced by the extent of coronary damage and vascular repair. The use of novel coronary cellular and soluble biomarkers, reactive to vascular damage and repair, are useful to predict the development of MACEs and prognosis.