USDA ARS Invasive Species and Pollinator Health 1 article published in JoVE Biology Assessing Agrochemical Risk to Mated Honey Bee Queens Julia D. Fine1, Kendall M. Torres2, Jamilyn Martin2, Gene E. Robinson2,3,4 1Invasive Species and Pollinator Health Research Unit, USDA-ARS, 2Carl R. Woese Institute for Genomic Biology, University of Illinois at Urbana-Champaign, 3Neuroscience Program, University of Illinois at Urbana-Champaign, 4Department of Entomology, University of Illinois at Urbana-Champaign This protocol was developed to enhance the understanding of how agrochemicals affect honey bee (Apis mellifera) reproduction by establishing methods to expose honey bee queens and their worker caretakers to agrochemicals in a controlled, laboratory setting and carefully monitoring their relevant responses.