3M Corporate Research Laboratory 1 article published in JoVE Immunology and Infection High Throughput Fluorometric Technique for Assessment of Macrophage Phagocytosis and Actin Polymerization Jana Ninković1,3, Sabita Roy1,2 1Department of Pharmacology, University of Minnesota, 2Department of Surgery, University of Minnesota, 33M Corporate Research Laboratory Here we present a protocol to quantify phagocytosis of fluorescent particles by adherent macrophage cell line using a fluorometric method. This method facilitates a high throughput quantification of particle internalization as well as the resulting actin polymerization.