ZeaVision LLC 1 article published in JoVE Medicine Measurement of Carotenoids in Perifovea using the Macular Pigment Reflectometer Juan C. Sanabria1, Jordan Bass1, Frank Spors1, Dennis L. Gierhart2, Pinakin Gunvant Davey1 1College of Optometry, Western University of Health Sciences, 2ZeaVision LLC We present a protocol to determine the levels of overall macular pigment, lutein, and zeaxanthin optical density in the central and parafoveal regions of the retina. The protocol includes a novel adjustable track system used to measure macular pigment optical density in the foveal eccentricity.