New Jersey City University 1 article published in JoVE Immunology and Infection A High-throughput Cre-Lox Activated Viral Membrane Fusion Assay to Identify Inhibitors of HIV-1 Viral Membrane Fusion Anthony M. Esposito1,2, Alexandra Y. Soare1, Foramben Patel1, Namita Satija1, Benjamin K. Chen1, Talia H. Swartz1 1Division of Infectious Diseases, Department of Medicine, Icahn School of Medicine at Mount Sinai, Immunology Institute, 2Department of Biology, New Jersey City University We describe a cell-based assay to report on HIV-1 fusion via the expression of green fluorescent protein detectable by flow cytometry or fluorescence microscopy. It can be used to test inhibitors of viral entry (specifically at the fusion step) in cell-free and cell-to-cell infection systems.