University of Nevada, Reno School of Medicine 6 articles published in JoVE Neuroscience Dosage-Adjusted Resistance Training in Mice with a Reduced Risk of Muscle Damage Morium Begam1, Neha Narayan1, Drew Mankowski1, Robert Camaj1, Nicholas Murphy1, Kevin Roseni1, Marie E. Pepin1, Jacob M. Blackmer1, Takako I. Jones2, Joseph A. Roche1 1Physical Therapy Program, Department of Health Care Sciences, Eugene Applebaum College of Pharmacy and Health Sciences, Wayne State University, 2Department of Pharmacology, University of Nevada, Reno School of Medicine The present protocol describes a unique technique called dosage-adjusted resistance training (DART), which can be incorporated into precision rehabilitation studies performed in small animals, such as mice. Biology Isolating and Imaging Live, Intact Pacemaker Regions of Mouse Renal Pelvis by Vibratome Sectioning Nathan Grainger1,2, Kenton M. Sanders1, Bernard T. Drumm1,3 1Department of Physiology & Cell Biology, University of Nevada, Reno School of Medicine, 2Department of Physiology & Membrane Biology, University of California School of Medicine, 3Department of Life & Health Sciences, Dundalk Institute of Technology The goal of this protocol is to isolate intact pacemaker regions of the mouse renal pelvis using vibratome sectioning. These sections can then be used for in situ Ca2+ imaging to elucidate Ca2+ transient properties of pacemaker cells and other interstitial cells in vibratome slices. Medicine A Decentralized (Ex Vivo) Murine Bladder Model with the Detrusor Muscle Removed for Direct Access to the Suburothelium during Bladder Filling Leonie Durnin1,2, Robert D. Corrigan1, Kenton M. Sanders1, Violeta N. Mutafova-Yambolieva1 1Department of Physiology and Cell Biology, University of Nevada Reno School of Medicine, 2Charles River Laboratories Inc. The detrusor-free bladder model enables direct access to the suburothelium to study local mechanisms for regulation of biologically active mediator availability in suburothelium/lamina propria during storage and voiding of urine. The preparation closely resembles filling of an intact bladder and allows pressure-volume studies to be performed without systemic influences. Biology Applications of Spatio-temporal Mapping and Particle Analysis Techniques to Quantify Intracellular Ca2+ Signaling In Situ Bernard T. Drumm1, Grant W. Hennig2, Salah A. Baker1, Kenton M. Sanders1 1Department of Physiology and Cell Biology, University of Nevada Reno School of Medicine, 2Department of Pharmacology, The Robert Larner, M.D. College of Medicine, University of Vermont Genetically encoded Ca2+ indicators (GECIs) have radically changed how in situ Ca2+ imaging is performed. To maximize data recovery from such recordings, appropriate analysis of Ca2+ signals is required. The protocols in this paper facilitate the quantification of Ca2+ signals recorded in situ using spatiotemporal mapping and particle-based analysis. Biology A Mouse Model of Intestinal Partial Obstruction Se Eun Ha*1, Lai Wei*1, Brian G. Jorgensen*1, Moon Young Lee*2, Paul J. Park1, Sandra M. Poudrier1, Seungil Ro1 1Department of Physiology and Cell Biology, University of Nevada, Reno School of Medicine, 2Department of Physiology, Wonkwang Digestive Disease Research Institute and Institute of Wonkwang Medical Science, School of Medicine, Wonkwang University Intestinal obstructions are a partial or complete blockage of the intestine that can cause severe abdominal pain, nausea, vomiting, and preventing the passage of stool. This procedure for creating intestinal partial obsructions in mice is reliable in studying the mechanisms underlying pathological cell growth and death in the intestine. Neuroscience Isolation and Cannulation of Cerebral Parenchymal Arterioles Paulo W. Pires1, Fabrice Dabertrand2, Scott Earley1 1Department of Pharmacology, University of Nevada School of Medicine, 2Department of Pharmacology, University of Vermont College of Medicine This manuscript describes a simple and reproducible protocol for isolation of intracerebral arterioles (a group of blood vessels encompassing parenchymal arterioles, penetrating arterioles and pre-capillary arterioles) from mice, to be used in pressure myography, immunofluorescence, biochemistry, and molecular studies.