The University of Texas Health Science Center at San Antonio 1 article published in JoVE Biochemistry Paramagnetic Relaxation Enhancement for Detecting and Characterizing Self-Associations of Intrinsically Disordered Proteins Courtney N. Johnson1, David S. Libich1 1Department of Biochemistry and Structural Biology and Greehey Children’s Cancer Research Institute, The University of Texas Health Science Center at San Antonio A protocol for the application of paramagnetic relaxation enhancement NMR spectroscopy to detect weak and transient inter- and intra-molecular interactions in intrinsically disordered proteins is presented.