Universidad Nacional de La Plata 1 article published in JoVE Neuroscience Micro-CT Imaging and Morphometric Analysis of Mouse Neonatal Brains Jimena Barbeito-Andrés1, Laura Andrini2, Mariana Vallejo-Azar1, Sara Seguel3, Jay Devine4, Benedikt Hallgrímsson4, Paula Gonzalez1 1Unidad Ejecutora de Estudios en Neurociencias y Sistemas Complejos (ENyS, CONICET-UNAJ-HEC), 2Facultad de Ciencias Médicas, Universidad Nacional de La Plata, 3Facultad de Ciencias Naturales y Museo, Universidad Nacional de La Plata, 4Department of Cell Biology and Anatomy, Cumming School of Medicine, University of Calgary This study describes the steps for obtaining high-resolution images of neonatal mouse brains by combining micro-computed tomography (micro-CT) and a contrast agent in ex vivo samples. We describe basic morphometric analyses to quantify brain size and shape in these images.