Grupo Boticario 3 articles published in JoVE Environment A 3D Culture Method of Spheroids of Embryonic and Liver Zebrafish Cell Lines Irisdoris Rodrigues de Souza1, Andrezza Di Pietro Micali Canavez2, Desiree Cigaran Schuck2, Viviana Stephanie Costa Gagosian1, Isisdoris Rodrigues de Souza1, Natália de Albuquerque Vita2, Edvaldo da Silva Trindade3, Marta Margarete Cestari4, Marcio Lorencini2, Daniela Morais Leme4 1Graduate Program in Genetics, Department of Genetics, Federal University of Paraná (UFPR), 2Safety of Product Department, Grupo Boticário, 3Department of Cell Biology, Federal University of Paraná (UFPR), 4Department of Genetics, Federal University of Paraná (UFPR) Here, we present an effective, easy, and fast 3D culture protocol for the formation of spheroids of two zebrafish (Danio rerio) cell lines: ZEM2S (embryo) and ZFL (normal hepatocyte). Environment Cytotoxicity Assays with Zebrafish Cell Lines Irisdoris Rodrigues de Souza1, Tainá Wilke Sivek1, Júlia Beatriz Vaz de Oliveira1, Andrezza Di Pietro Micali Canavez2, Natália de Albuquerque Vita2, Desiree Cigaran Schuck2, Isisdoris Rodrigues de Souza1, Marta Margarete Cestari3, Marcio Lorencini2, Daniela Morais Leme3 1Graduate Program in Genetics, Department of Genetics, Federal University of Paraná (UFPR), 2Safety of Product Department, Grupo Boticário, 3Department of Genetics, Federal University of Paraná (UFPR) This protocol presents commonly used cytotoxicity assays (Alamar Blue [AB], CFDA-AM, Neutral Red, and MTT assays) adapted for the assessment of cytotoxicity in zebrafish embryo (ZEM2S) and liver (ZFL) cell lines in 96-well plates. Chemistry An Intestine/Liver Microphysiological System for Drug Pharmacokinetic and Toxicological Assessment Talita Miguel Marin1, Nathalia de Carvalho Indolfo4, Silvana Aparecida Rocco1, Murilo de Carvalho1,2, Marilia Meira Dias1, Graziele Izalina Vasconcelos Bento1, Leandro Oliveira Bortot1, Desirée Cigaran Schuck3, Márcio Lorencini3, Eduardo Pagani1 1Brazilian Biosciences National Laboratory (LNBio), Brazilian Center for Research in Energy and Materials (CNPEM), 2Brazilian Synchrotron Light Laboratory (LNLS), Brazilian Center for Research in Energy and Materials (CNPEM), 3Grupo Boticário, 4Institute of Biology, University of Campinas We exposed a microphysiological system (MPS) with intestine and liver organoids to acetaminophen (APAP). This article describes the methods for organoid production and APAP pharmacokinetic and toxicological property assessments in the MPS. It also describes the tissue functionality analyses necessary to validate the results.