JoVE Journal
Immunology and Infection
Immunology and Infection
A subscription to JoVE is required to view this content. Sign in or start your free trial.
Chapters
Summary
This co-immunoprecipitation protocol allows to study the interaction between the influenza A virus nucleoprotein and the antiviral Mx1 protein in human cells. The protocol emphasizes the importance of N-ethylmaleimide for successful co-immunoprecipitation of Mx1 and influenza A virus nucleoprotein.