JoVE Journal
Chemistry
Chemistry
A subscription to JoVE is required to view this content. Sign in or start your free trial.
Chapters
Summary
This protocol details the steps, costs, and equipment necessary to generate E. coli-based cell extracts and implement in vitro protein synthesis reactions within 4 days or less. To leverage the flexible nature of this platform for broad applications, we discuss reaction conditions that can be adapted and optimized.