JoVE Journal
Immunology and Infection
Immunology and Infection
This content is Open Access.
Chapters
Summary
We present a protocol for using a protein microarray constructed by printing influenza antigens onto nitrocellulose-coated slides to simultaneously probe serum for multiple antibody isotypes against over 250 antigens from different virus strains, thus allowing measurement of the breadth of serum antibodies across virus subtypes.