Nansha College Preparatory Academy 1 article published in JoVE Bioengineering Construction of a Multilayered Mesenchymal Stem Cell Sheet with a 3D Dynamic Culture System Yingwei Wang*1,2, Cheng Lu*1,2, Chengzhi He3, Baoxin Chen1,2, Youling Zheng1,2, Junming Zheng1,2, Jianhua Zhang4, Zheng Wu1,2 1Key Laboratory for Regenerative Medicine, Ministry of Education, Jinan University, 2Department of Developmental and Regenerative Biology, Jinan University, 3Nansha College Preparatory Academy, 4Department of Cardiology, First Affiliated Hospital of Jinan University This article provides an efficient and feasible method for constructing multilayered stem cell sheets with favorable stem cell property.