Unita di Perugia 1 article published in JoVE Bioengineering Preparation of Extracellular Matrix Protein Fibers for Brillouin Spectroscopy Ryan S. Edginton1, Sara Mattana2, Silvia Caponi2,3, Daniele Fioretto2, Ellen Green1, C. Peter Winlove1, Francesca Palombo1 1School of Physics and Astronomy, University of Exeter, 2Department of Physics and Geology, University of Perugia, 3Istituto Officina dei Materiali del CNR, Unità di Perugia We present a protocol for the application of Brillouin light scattering spectroscopy to elastin and trypsin-purified type I collagen fibers of the extracellular matrix to extract their full elastic properties.