Harvard School of Engineering and Applied Sciences 1 article published in JoVE Biology Intraductal Injection for Localized Drug Delivery to the Mouse Mammary Gland Silva Krause1, Amy Brock2, Donald E. Ingber1,2,3 1 A protocol for the non-invasive intraductal delivery of aqueous reagents to the mouse mammary gland is described. The method takes advantage of localized injection into the nipples of mammary glands targeting mammary ducts specifically. This technique is adaptable for a variety of compounds including siRNA, chemotherapeutic agents and small molecules.