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Immunology and Infection

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Quantifying Human Monocyte Chemotaxis In Vitro and Murine Lymphocyte Trafficking In Vivo

Article DOI: 10.3791/56218-v • 08:38 min • October 30th, 2017
October 30th, 2017 •
1, 1, 1
1Laboratory of Biochemical Genetics and Metabolism, The Rockefeller University

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Protocols for quantitative assessment of lymphocyte chemotaxis and migration are important tools for immunology research. Here, an in vitro protocol is described that permits real-time, multiplexed evaluation of cell migration, as well as a complementary in vivo technique enabling tracking of native cells to spleen.

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Quantify Monocyte Chemotaxis In Vitro Murine Lymphocyte Trafficking In Vivo Immunology Serum Factors Immune Cell Chemotaxis Quantitative Measurements Multiplex Measurements In Vivo Technique Recipient Animal Label THP-1 Cells Fluorescent Dye Cell Culture Dish Complete Medium Incubating HBSS Buffered With HEPES And BSA MCP-1 Water Supplemented With BSA Porous Inserts Fluorescence Measurements
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