In JoVE (1)
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Articles by Aaron A. May-Zhang in JoVE
Optimization of Laser-Capture Microdissection for the Isolation of Enteric Ganglia from Fresh-Frozen Human Tissue Aaron A. May-Zhang1, Karen K Deal1, E. Michelle Southard-Smith1 1Vanderbilt University Medical Center The goal of this protocol is to obtain high-integrity RNA samples from enteric ganglia isolated from unfixed, freshly-resected human intestinal tissue using laser capture microdissection (LCM). This protocol involves preparing flash-frozen samples of human intestinal tissue, cryosectioning, ethanolic staining and dehydration, LCM, and RNA extraction.