Articles by Agnes S. Pascual in JoVE

• Developmental Biology

  
  Zebrafish Keratocyte Explants to Study Collective Cell Migration and Reepithelialization in Cutaneous Wound Healing Jose L. Rapanan¹, Agnes S. Pascual¹, Chandana K. Uppalapati², Kimbal E. Cooper¹, Kathryn J. Leyva², Elizabeth E. Hull^{1 1}Biomedical Sciences Program, Midwestern University, ²Department of Microbiology & Immunology, Midwestern University Zebrafish keratocytes migrate in cell sheets from explants and provide an in vitro model for the study of the mechanisms of collective cell migration in the context of epithelial wound healing. These protocols detail an effective way to establish primary explant cultures for use in collective cell migration assays.

• Medicine

  
  Establishment of a Clinic-based Biorepository Sarah E. Belden¹, Chandana K. Uppalapati², Agnes S. Pascual³, McKale R. Montgomery³, Kathryn J. Leyva², Elizabeth E. Hull³, Richard L. Averitte^{1 1}Affiliated Dermatology & Affiliated Laboratories, Midwestern University Osteopathic Postdoctoral Training Institute, Midwestern University, ²Department of Microbiology & Immunology, Arizona College of Osteopathic Medicine, Midwestern University, ³Biomedical Sciences Program, College of Health Sciences, Midwestern University Cutaneous tumors are often discarded following Mohs micrographic surgery. A protocol is described here that enables clinical support staff to effectively process and store cutaneous tumor (e.g., squamous cell carcinoma, basal cell carcinoma, and melanoma) samples for downstream laboratory applications without interfering with clinical operations.