In JoVE (1)
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Articles by Ahmad Essawy in JoVE
Nutrient Regulation by Continuous Feeding for Large-scale Expansion of Mammalian Cells in Spheroids Bradley P. Weegman1, Ahmad Essawy2, Peter Nash2, Alexandra L. Carlson2, Kristin J. Voltzke3, Zhaohui Geng2, Marjan Jahani2, Benjamin B. Becker2, Klearchos K. Papas4, Meri T. Firpo2 1Radiology, University of Minnesota, 2Medicine, University of Minnesota, 3School of Public Health, University of Minnesota, 4Surgery, University of Arizona Nutrient regulation using continuous growth adjusted feeding improves growth rates of mammalian cell spheroids compared to intermittent batch feeding for cultures in stirred suspension bioreactors. This study demonstrates the methods required for establishing simple adjusted rate fed cultures.