Articles by Ahmad Essawy in JoVE

• Bioengineering

  
  Nutrient Regulation by Continuous Feeding for Large-scale Expansion of Mammalian Cells in Spheroids Bradley P. Weegman¹, Ahmad Essawy², Peter Nash², Alexandra L. Carlson², Kristin J. Voltzke³, Zhaohui Geng², Marjan Jahani², Benjamin B. Becker², Klearchos K. Papas⁴, Meri T. Firpo^{2 1}Radiology, University of Minnesota, ²Medicine, University of Minnesota, ³School of Public Health, University of Minnesota, ⁴Surgery, University of Arizona Nutrient regulation using continuous growth adjusted feeding improves growth rates of mammalian cell spheroids compared to intermittent batch feeding for cultures in stirred suspension bioreactors. This study demonstrates the methods required for establishing simple adjusted rate fed cultures.