Other Publications (1)
Articles by Akiko Nakamasu in JoVE
Perfusable Vascular Network with a Tissue Model in a Microfluidic Device Yuji Nashimoto1, Yukako Teraoka1, Ramin Banan Sadeghian1, Akiko Nakamasu2, Yuichiro Arima3, Sanshiro Hanada3, Hidetoshi Kotera1, Koichi Nishiyama3, Takashi Miura2, Ryuji Yokokawa1 1Department of Micro Engineering, Kyoto University, 2Graduate School of Medical Sciences, Kyushu University, 3International Research Center for Medical Sciences (IRCMS), Kumamoto University The protocol describes how to engineer a perfusable vascular network in a spheroid. The spheroid's surrounding microenvironment is devised to induce angiogenesis and connect the spheroid to the microchannels in a microfluidic device. The method allows the perfusion of the spheroid, which is a long-awaited technique in three-dimensional cultures.
Other articles by Akiko Nakamasu on PubMed
High-frequency Affine Mechanics and Nonaffine Relaxation in a Model Cytoskeleton Physical Review. E, Statistical, Nonlinear, and Soft Matter Physics. | Pubmed ID: 24827282 The cytoskeleton is a network of crosslinked, semiflexible filaments, and it has been suggested that it has properties of a glassy state. Here we employ optical-trap-based microrheology to apply forces to a model cytoskeleton and measure the high-bandwidth response at an anterior point. Simulating the highly nonlinear and anisotropic stress-strain propagation assuming affinity, we found that theoretical predictions for the quasistatic response of semiflexible polymers are only realized at high frequencies inaccessible to conventional rheometers. We give a theoretical basis for determining the frequency when both affinity and quasistaticity are valid, and we discuss with experimental evidence that the relaxations at lower frequencies can be characterized by the experimentally obtained nonaffinity parameter.