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Articles by Alejandra Hernandez-Segura in JoVE
Induction and Validation of Cellular Senescence in Primary Human Cells Alejandra Hernandez-Segura1, Simone Brandenburg1, Marco Demaria1 1European Research Institute for the Biology of Aging, University of Groningen, University Medical Center Groningen Here, we discuss a series of protocols for induction and validation of cellular senescence in cultured cells. We focus on different senescence-inducing stimuli and describe the quantification of common senescence-associated markers. We provide technical details using fibroblasts as a model, but the protocols can be adapted to various cellular models.