Other Publications (1)
Articles by Alexa Levinson in JoVE
Electrically Conductive Scaffold to Modulate and Deliver Stem Cells Byeongtaek Oh1, Alexa Levinson1, Vivek Lam1, Shang Song1, Paul George1,2 1Department of Neurology and Neurological Sciences, Stanford University School of Medicine, 2Stanford Stroke Center and Stanford University School of Medicine This protocol describes fabrication of a cell culture system to allow seeding of stem cells on a conductive polymer scaffold for in vitro electrical stimulation and subsequent in vivo implantation of the stem cell-seeded scaffold using a minimally invasive technique.
Other articles by Alexa Levinson on PubMed
Electrical Preconditioning of Stem Cells with a Conductive Polymer Scaffold Enhances Stroke Recovery Biomaterials. | Pubmed ID: 28719819 Exogenous human neural progenitor cells (hNPCs) are promising stroke therapeutics, but optimal delivery conditions and exact recovery mechanisms remain elusive. To further elucidate repair processes and improve stroke outcomes, we developed an electrically conductive, polymer scaffold for hNPC delivery. Electrical stimulation of hNPCs alters their transcriptome including changes to the VEGF-A pathway and genes involved in cell survival, inflammatory response, and synaptic remodeling. In our experiments, exogenous hNPCs were electrically stimulated (electrically preconditioned) via the scaffold 1 day prior to implantation. After in vitro stimulation, hNPCs on the scaffold are transplanted intracranially in a distal middle cerebral artery occlusion rat model. Electrically preconditioned hNPCs improved functional outcomes compared to unstimulated hNPCs or hNPCs where VEGF-A was blocked during in vitro electrical preconditioning. The ability to manipulate hNPCs via a conductive scaffold creates a new approach to optimize stem cell-based therapy and determine which factors (such as VEGF-A) are essential for stroke recovery.