Articles by Alexander F. Mason in JoVE
Synthèse des bioconjugués protéiques Alexander F. Mason1, Pall Thordarson1 1School of Chemistry, The Australian Centre for Nanomedicine and the ARC Centre of Excellence in Convergent Bio-Nano Science and Technology, The University of New South Wales Ce protocole détaille les étapes importantes nécessaires pour la bioconjugaison d'une cystéine contenant la protéine à un maléimide, y compris la purification des réactifs, des conditions de réaction, la purification de bioconjugués et bioconjugué de caractérisation.
Other articles by Alexander F. Mason on PubMed
Optimising the Synthesis, Polymer Membrane Encapsulation and Photoreduction Performance of Ru(II)- and Ir(III)-bis(terpyridine) Cytochrome C Bioconjugates Organic & Biomolecular Chemistry. Jul, 2013 | Pubmed ID: 23715338 Ruthenium(II) and iridium(III) bis(terpyridine) complexes were prepared with maleimide functionalities in order to site-specifically modify yeast iso-1 cytochrome c possessing a single cysteine residue available for modification (CYS102). Single X-ray crystal structures were solved for aniline and maleimide Ru(II) 3 and Ru(II) 4, respectively, providing detailed structural detail of the complexes. Light-activated bioconjugates prepared from Ru(II) 4 in the presence of tris(2-carboxyethyl)-phosphine (TCEP) significantly improved yields from 6% to 27%. Photoinduced electron transfer studies of Ru(II)-cyt c in bulk solution and polymer membrane encapsulated specimens were performed using EDTA as a sacrificial electron donor. It was found that membrane encapsulation of Ru(II)-cyt c in PS140-b-PAA48 resulted in a quantum efficiency of 1.1 ± 0.3 × 10(-3), which was a two-fold increase relative to the bulk. Moreover, Ir(III)-cyt c bioconjugates showed a quantum efficiency of 3.8 ± 1.9 × 10(-1), equivalent to a ∼640-fold increase relative to bulk Ru(II)-cyt c.
Exceptionally Strong Hydrogels Through Self-assembly of an Indole-capped Dipeptide Chemical Communications (Cambridge, England). Dec, 2014 | Pubmed ID: 25354784 The synthesis of a new hydrogelator with an indole capping group, 1, is reported. 1 forms exceptionally strong hydrogels in a variety of environments, with values for the storage modulus G' amongst the highest reported for supramolecular hydrogels. These gels exhibit strong bundling characteristics, which gives the high values for G' observed. Cell viability studies show that at low concentrations, 1 is biocompatible, however upon self-assembly at higher concentrations, cytotoxic effects are observed.