Other Publications (1)
Articles by Alexander Sobczak in JoVE
In vivo mikroinjektion och elektroporation av Mus Testikel Marten Michaelis1, Alexander Sobczak1, Joachim M. Weitzel1 1Institute of Reproductive Biology, Leibniz Institute for Farm Animal Biology (FBN) I denna artikel beskrivs mikroinjektion och elektroporering av mus testiklar in vivo som en transfektion teknik för testikel musceller att studera unika processer av spermatogenesen. Den presenterade protokollet involverar stegen att preparatet glaskapillär, mikroinjektion via efferenta kanalen, och transfektering genom elektroporation.
Other articles by Alexander Sobczak on PubMed
High-fertility Phenotypes: Two Outbred Mouse Models Exhibit Substantially Different Molecular and Physiological Strategies Warranting Improved Fertility Reproduction (Cambridge, England). 2014 | Pubmed ID: 24248751 Animal models are valuable tools in fertility research. Worldwide, there are more than 400 transgenic or knockout mouse models available showing a reproductive phenotype; almost all of them exhibit an infertile or at least subfertile phenotype. By contrast, animal models revealing an improved fertility phenotype are barely described. This article summarizes data on two outbred mouse models exhibiting a 'high-fertility' phenotype. These mouse lines were generated via selection over a time period of more than 40 years and 161 generations. During this selection period, the number of offspring per litter and the total birth weight of the entire litter nearly doubled. Concomitantly with the increased fertility phenotype, several endocrine parameters (e.g. serum testosterone concentrations in male animals), physiological parameters (e.g. body weight, accelerated puberty, and life expectancy), and behavioral parameters (e.g. behavior in an open field and endurance fitness on a treadmill) were altered. We demonstrate that the two independently bred high-fertility mouse lines warranted their improved fertility phenotype using different molecular and physiological strategies. The fertility lines display female- as well as male-specific characteristics. These genetically heterogeneous mouse models provide new insights into molecular and cellular mechanisms that enhance fertility. In view of decreasing fertility in men, these models will therefore be a precious information source for human reproductive medicine. Translated abstract A German translation of abstract is freely available at http://www.reproduction-online.org/content/147/4/427/suppl/DC1.