Other Publications (1)
Articles by Ali Osman Gürol in JoVE
Intraportal Transplantation of Pancreatic Islets in Mouse Model Rahul Khatri1, Birte Hussmann1, Divya Rawat1, Ali Osman Gürol2, Thomas Linn1 1Third Medical Department, Clinical Research Lab, 2Istanbul University Department of Immunology, Institute of Experimental Medicine (DETAE) Pancreatic islet transplantation is a way to achieve normoglycemia in type 1 diabetes. This article focuses on the transplantation technique through the intraportal route to maintain normal glucose levels in diabetic mice.
Other articles by Ali Osman Gürol on PubMed
The Synergistic Effect of ω3 and Vit D3 on Glycemia and TNF-α in Islet Transplantation Cellular and Molecular Biology (Noisy-le-Grand, France). | Pubmed ID: 26828994 The current treatment of type 1 diabetes consists of insulin administration. Transplantation of islets of Langerhans is considered very favorable because the full effect of insulin treatment cannot be obtained in severe cases. Although agents such as omega-3 (ω3) and vitamin D3 (Vit D3) are known to contribute to the success of islet allo-transplantation (ITX), in this study we aimed to experimentally determine their effects on glycemia and TNF-α production. Wistar albino rats, which were used as recipients, were given ω3, Vit D3, and islets by gavage, and intraperitoneal- and intraportal injections, respectively. Daclizumab (DAC) was used for immunosuppression. Glycemia levels decreased in rats treated with ω3 and vit D3. TNF-α increased in all groups due to application of STZ. After ITX (day +1), the weakest increase was observed in the ω3 + Vit D3 group. In the ITX+DAC group, compared with that of ITX only, DAC was shown to decrease levels of TNF- α following ITX, only in control group, however, similar levels of TNF-α were observed in other groups. The values in the treated groups were already lower than those of the controls in the ITX group and also remained almost equal in the ITX+DAC group. We suggest that the use of ω3 and Vit D3 together will improve the pro-inflammatory aspect encountered during and after ITXs, and contribute to the reduction of the dose of immunosuppressants in these procedures.