Articles by Alice Bertero in JoVE
检测荧光纳米粒子的相互作用与原发性免疫细胞亚群流式细胞仪的 Olimpia Gamucci1, Alice Bertero1,2, Maria Ada Malvindi3, Stefania Sabella3, Pier Paolo Pompa3, Barbara Mazzolai1, Giuseppe Bardi1 1Center for Micro-BioRobotics @SSSA, Istituto Italiano di Tecnologia, 2Department of Biology, University of Pisa, 3Center for Biomolecular Nanotechnologies @UniLe, Istituto Italiano di Tecnologia 分析与免疫细胞的流式细胞仪检测亚组人群纳米粒子的相互作用。
Other articles by Alice Bertero on PubMed
Surface Functionalisation Regulates Polyamidoamine Dendrimer Toxicity on Blood-brain Barrier Cells and the Modulation of Key Inflammatory Receptors on Microglia Nanotoxicology. Mar, 2014 | Pubmed ID: 23298388 Dendrimers are branched polymers with spherical morphology. Their tuneable chemistry and surface modification make them valuable nanomaterials for biomedical applications. In view of possible dendrimer uses as brain-aimed nanocarriers, the authors studied amine- and lipid-functionalised (G4) polyamidoamine (PAMAM) biocompatibility with cell population forming the blood-brain barrier (BBB). Both amine-PAMAM and lipid-PAMAM dendrimers were able to enter endothelial and primary neural cells. However, only amine-PAMAM damaged cell membranes in a dose-dependent manner. Transmission electron microscopy evidenced the ability of dendrimers to precipitate salts and serum components present in culture medium that slightly increased toxicity of the amine-PAMAM. Amine- and lipid-PAMAM were both able to cross the BBB and differently induced CD11b and CCR2 overexpression on primary CX3CR1-GFP murine microglia in vitro. These data emphasise the role of dendrimer surface functionalisation in toxicity and neural immune cell activation, raising concerns about possible neuroinflammatory reactions.