In JoVE (1)
Other Publications (1)
Articles by Amanda Kauffman in JoVE
C. elegans Positieve Butanon Learning, op korte termijn en lange termijn geheugen Associatieve Assays Amanda Kauffman1, Lance Parsons2, Geneva Stein1, Airon Wills1, Rachel Kaletsky1, Coleen Murphy1 1Department of Molecular Biology, Lewis-Sigler Institute for Integrative Genomics, Princeton University, 2Lewis-Sigler Institute for Integrative Genomics, Princeton University Hier beschrijven we methoden om te testen C. elegans associatief leren en korte-en lange termijn associatief geheugen. Deze populatie testen maken gebruik van de wormen mogelijkheden om chemotax in de richting van vluchtige geur-, en vormen positieve associaties bij de paring voedsel met de chemoattractant butanon. Verhoging van het aantal van conditionering periodes leidt tot lange-termijn geheugen.
Other articles by Amanda Kauffman on PubMed
Insulin Signaling and Dietary Restriction Differentially Influence the Decline of Learning and Memory with Age PLoS Biology. May, 2010 | Pubmed ID: 20502519 Of all the age-related declines, memory loss is one of the most devastating. While conditions that increase longevity have been identified, the effects of these longevity-promoting factors on learning and memory are unknown. Here we show that the C. elegans Insulin/IGF-1 receptor mutant daf-2 improves memory performance early in adulthood and maintains learning ability better with age but, surprisingly, demonstrates no extension in long-term memory with age. By contrast, eat-2 mutants, a model of Dietary Restriction (DR), exhibit impaired long-term memory in young adulthood but maintain this level of memory longer with age. We find that crh-1, the C. elegans homolog of the CREB transcription factor, is required for long-term associative memory, but not for learning or short-term memory. The expression of crh-1 declines with age and differs in the longevity mutants, and CREB expression and activity correlate with memory performance. Our results suggest that specific longevity treatments have acute and long-term effects on cognitive functions that decline with age through their regulation of rate-limiting genes required for learning and memory.