Other Publications (1)
Articles by Amelia R. Adelsperger in JoVE
Use of a Low-flow Digital Anesthesia System for Mice and Rats Amelia R. Adelsperger1, Krista J. Bigiarelli-Nogas2, Irina Toore2, Craig J. Goergen1 1Weldon School of Biomedical Engineering, Purdue University, 2Pre-Clinical Research and Development, Kent Scientific Corporation Here, we present a protocol to more safely and efficiently administer anesthetic gas to mice using a digital, low flow anesthesia system utilizing a syringe-driven direct injection vaporizer.
Other articles by Amelia R. Adelsperger on PubMed
Comparison of Traditional and Integrated Digital Anesthetic Vaporizers Journal of the American Association for Laboratory Animal Science : JAALAS. Nov, 2015 | Pubmed ID: 26632785 Recent efforts have focused on mitigating anesthetic gas emissions during laboratory animal experiments. A recently developed, digitally controlled, integrated digital vaporizer (IDV) using a syringe pump has been designed to use and administer anesthetic gas to mice and rats more efficiently. The entire IDV system can be placed on a laboratory bench, requires fewer charcoal filters to act as passive scavengers when used at a low gas flow rate, and does not need compressed gas to operate, a requirement for traditional passive systems. The objective of this study was to compare isoflurane usage between a traditional vaporizer (TdV) and an IDV system at both the same settings and those recommended by the manufacturer. We used 10 C57BL/6 male mice and administered isoflurane through either nose cones or tracheal tubes connected to a pulsatile ventilator. The results showed that isoflurane usage is highly dependent on the flow rate of the carrier gas, but the IDV system was more precise and handled low flow rates (150 mL/min) better than did the TdV system. We observed only slight differences in heart rate, respiration rate, core body temperature, time to loss of the righting reflex, and recovery time between group averages for both systems when set to manufacturer-recommended settings. Although observed decreased levels of waste anesthetic gas at low flow rates are expected from the IDV system, further work is needed to assess environmental anesthetic gas levels and exposure to laboratory personnel.