Other Publications (1)
Articles by Ana Marie S. Palanca in JoVE
Optogenetic Activation of Zebrafish Somatosensory Neurons using ChEF-tdTomato Ana Marie S. Palanca1, Alvaro Sagasti1 1Department of Molecular, Cell, and Developmental Biology, University of California, Los Angeles Optogenetic techniques have made it possible to study the contribution of specific neurons to behavior. We describe a method in larval zebrafish for activating single somatosensory neurons expressing a channelrhodopsin variant (ChEF) with a diode-pumped solid state (DPSS) laser and recording the elicited behaviors with a high-speed video camera.
Other articles by Ana Marie S. Palanca on PubMed
New Transgenic Reporters Identify Somatosensory Neuron Subtypes in Larval Zebrafish Developmental Neurobiology. Feb, 2013 | Pubmed ID: 22865660 To analyze somatosensory neuron diversity in larval zebrafish, we identified several enhancers from the zebrafish and pufferfish genomes and used them to create five new reporter transgenes. Sequential deletions of three of these enhancers identified small sequence elements sufficient to drive expression in zebrafish trigeminal and Rohon-Beard (RB) neurons. One of these reporters, using the Fru.p2x3-2 enhancer, highlighted a somatosensory neuron subtype that expressed both the p2rx3a and pkcÎ± genes. Comparison with a previously described trpA1b reporter revealed that it highlighted the same neurons as the Fru.p2x3-2 reporter. To determine whether neurons of this subtype possess characteristic peripheral branching morphologies or central axon projection patterns, we analyzed the morphology of single neurons. Surprisingly, although these analyses revealed diversity in peripheral axon branching and central axon projection, PKCÎ±/p2rx3a/trpA1b-expressing RB cells did not possess obvious characteristic morphological features, suggesting that even within this molecularly defined subtype, individual neurons may possess distinct properties. The new transgenes created in this study will be powerful tools for further characterizing the molecular, morphological, and developmental diversity of larval somatosensory neurons. Â© 2012 Wiley Periodicals, Inc., 2013.