In JoVE (1)
Other Publications (1)
Articles by Ana Marin Navarro in JoVE
Integration Free Derivation of Human Induced Pluripotent Stem Cells Using Laminin 521 Matrix Elias Uhlin1, Ana Marin Navarro1,2, Harriet Rönnholm1, Kelly Day1, Malin Kele1, Anna Falk1 1Department of Neuroscience, Karolinska Institutet, 2Department of Microbiology, Tumor and Cell Biology, Karolinska Institutet Robust derivation of human induced pluripotent stem (hiPS) cells was achieved by using non-integrating Sendai virus (SeV) vector mediated reprogramming of dermal fibroblasts. hiPS cell maintenance and clonal expansion was performed using xeno-free and chemically defined culture conditions with recombinant human laminin 521 (LN-521) matrix and Essential E8 (E8) Medium.
Other articles by Ana Marin Navarro on PubMed
Modulation of P53 C-terminal Acetylation by Mdm2, P14ARF, and Cytoplasmic SirT2 Molecular Cancer Therapeutics. Apr, 2013 | Pubmed ID: 23416275 Acetylation of C-terminal lysine residues in the p53 tumor suppressor is associated with increased stability and transcription factor activity. The function, protein level, and acetylation of p53 are downregulated by mdm2, which in its turn is inhibited by the p14(ARF) tumor suppressor. Here, we show that p14(ARF) increases the level of p53 acetylated at lysine 382 in a nuclear chromatin-rich fraction. Unexpectedly, this accumulation of p53AcK382 is dramatically enhanced in the presence of ectopic mdm2. In light of these observations, we propose that p14(ARF) increases the binding of p53-mdm2 complexes to chromatin, thereby limiting the access of protein deacetylases to p53. Supporting this notion, we show that p53AcK382 can be deacetylated in the cytoplasm and that sirtuin SirT2 catalyzes this reaction. These results help understand why inhibition of both SirT1 and SirT2 is needed to achieve effective activation of p53 by small-molecule sirtuin inhibitors.