Articles by Andrea Li Bassi in JoVE
Fabrication of Nano-engineered Transparent Conducting Oxides by Pulsed Laser Deposition Paolo Gondoni1, Matteo Ghidelli1, Fabio Di Fonzo1,2, Andrea Li Bassi1,2, Carlo S. Casari1 1Department of Energy and NEMAS - Center for NanoEngineered Materials and Surfaces, Politecnico di Milano, 2Center for Nano Science and Technology, Instituto Italiano di Tecnologia We describe the experimental method to deposit nanostructured oxide thin films by nanosecond Pulsed Laser Deposition (PLD) in the presence of a background gas. By using this method Al-doped ZnO (AZO) films, from compact to hierarchically structured as nano-tree forests, can be deposited.
Other articles by Andrea Li Bassi on PubMed
Au-Ag Template Stripped Pattern for Scanning Probe Investigations of DNA Arrays Produced by Dip Pen Nanolithography Langmuir : the ACS Journal of Surfaces and Colloids. Nov, 2008 | Pubmed ID: 18950214 We report on DNA arrays produced by dip pen nanolithography (DPN) on a novel Au-Ag micropatterned template stripped surface. DNA arrays have been investigated by atomic force microscopy (AFM) and scanning tunneling microscopy (STM) showing that the patterned template stripped substrate enables easy retrieval of the DPN-functionalized zone with a standard optical microscope permitting multi-instrument and multitechnique local detection and analysis. Moreover the smooth surface of the Au squares ( approximately 5-10 A roughness) allows AFM/STM to be sensitive to the hybridization of the oligonucleotide array with label-free target DNA. Our Au-Ag substrates, combining the retrieving capabilities of the patterned surface with the smoothness of the template stripped technique, are candidates for the investigation of DPN nanostructures and for the development of label-free detection methods for DNA nanoarrays based on the use of scanning probes.
Nanostructured TiOâ‚‚ Thin Films for Phosphoproteomics Studies with MALDI Mass Spectrometry Methods in Molecular Biology (Clifton, N.J.). 2011 | Pubmed ID: 21948414 Alterations in protein phosphorylation, a posttranslational modification (PTM) that regulates many -processes in living cells, is a fundamental mechanism of many diseases, including cancer. Phosphoproteomics, with the combined use of affinity chromatography and electrospray ionization (ESI) or matrix-assisted laser desorption/ionization (MALDI) mass spectrometry, is shedding light into phosphorylation signaling pathways at the proteome level and helps to solve difficulties related to sample complexity and phosphopeptide enrichment. One of the most frequent and efficient methods used to enrich samples for the phosphorylated components is titanium dioxide chromatography. Titanium dioxide has a high affinity for phosphopeptides and can also be selective in specific experimental conditions. Here, we describe a protocol for the use of a MALDI plate covered with titanium dioxide nanostructured film, a device developed for a rapid and efficient study of phosphorylated peptides.