Other Publications (1)
Articles by Andreas Behring in JoVE
In Vitro Synthesis of Modified mRNA for Induction of Protein Expression in Human Cells Meltem Avci-Adali1, Andreas Behring1, Heidrun Steinle1, Timea Keller1, Stefanie Krajeweski1, Christian Schlensak1, Hans P. Wendel1 1Department of Thoracic, Cardiac, and Vascular Surgery, University Hospital Tuebingen In this video article, we describe the in vitro synthesis of modified mRNA for induction of protein expression in cells.
Other articles by Andreas Behring on PubMed
Optimized Conditions for Successful Transfection of Human Endothelial Cells with in Vitro Synthesized and Modified MRNA for Induction of Protein Expression Journal of Biological Engineering. 2014 | Pubmed ID: 24581116 The induction of protein synthesis by exogenous delivery of coding synthetic mRNA in desired cells is an auspicious strategy in the fields of basic cell biology, regenerative medicine, treatment of diseases, and reprogramming of cells. Here, we produced modified messenger RNA (mRNA) with reduced immune activation potential and increased stability and performed transfection experiments with different cells, HEK293 cells, BJ fibroblasts, and endothelial cells (ECs).