In JoVE (1)
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Articles by Andrew R. Peleman in JoVE
Imaging Plasma Membrane Deformations With pTIRFM Daniel R. Passmore1, Tejeshwar C. Rao1, Andrew R. Peleman1, Arun Anantharam1 1Department of Biological Sciences, Wayne State University Polarization-based Total Internal Reflection Fluorescence Microscopy (pTIRFM) enables real-time detection of cell membrane dynamics. This article describes the implementation of pTIRFM for the study of membrane remodeling during regulated exocytosis. The technique is generalizable to other processes in cell biology that directly or indirectly involve changes in membrane shape.