Articles by Anna Lagunas in JoVE
基于状的不均匀 Nanopatterns 对局部控制表面粘附的研究--一种直接软骨分化的方法 Ignasi Casanellas1,2, Anna Lagunas3,1, Iro Tsintzou1, Yolanda Vida4,5, Daniel Collado4,5, Ezequiel Pérez-Inestrosa4,5, Cristina Rodríguez-Pereira6, Joana Magalhaes3,6, Pau Gorostiza1,3,7, José A. Andrades8,3, José Becerra8,3,5, Josep Samitier1,3,2 1Institute for Bioengineering of Catalonia (IBEC), The Barcelona Institute of Science and Technology (BIST), 2Department of Engineering Electronics, University of Barcelona (UB), 3Networking Biomedical Research Center (CIBER), 4Instituto de Investigacin Biomédica de Málaga (IBIMA), Department of Organic Chemistry, Universidad de Málaga (UMA), 5Andalusian Centre for Nanomedicine and Biotechnology-BIONAND, 6Unidad de Bioingeniería Tisular y Terapia Celular (GBTTC-CHUAC), Grupo de Reumatolog ía, Instituto de Investigación Biomèdica de A Coruña (INIBIC), Complexo Hospitalario Universitario de A Coruña (CHUAC), Sergas, Universidade da Coruña (UDC), 7Institució Catalana de Recerca i Estudis Avançats (ICREA), 8Instituto de Investigación Biomédica de Málaga (IBIMA), Department of Cell Biology, Genetics and Physiology, Universidad de Málaga (UMA) 本文介绍了一种获得基于状的不均匀 nanopatterns 的方法, 该法允许对局部精氨酸-甘氨酸 (RGD) 的表面密度进行纳米控制, 并将其用于细胞黏附和软骨分化的研究。
Other articles by Anna Lagunas on PubMed
Cell Adhesion and Focal Contact Formation on Linear RGD Molecular Gradients: Study of Non-linear Concentration Dependence Effects Nanomedicine : Nanotechnology, Biology, and Medicine. | Pubmed ID: 21856276 Cell adhesion onto bioengineered surfaces is affected by a number of variables, including the former substrate derivatization process. In this investigation, we studied the correlation between cell adhesion and cell-adhesive ligand surface concentration and organization due to substrate modification. For this purpose, Arg-Gly-Asp (RGD) gradient surfaces were created on poly(methyl methacrylate) substrates by continuous hydrolysis and were then grafted with biotin-PEG-RGD molecules. Cell culture showed that adhesion behavior changes in a nonlinear way in the narrow range of RGD surface densities assayed (2.8 to 4.4 pmol/cm(2)), with a threshold value of 4.0 pmol/cm(2) for successful cell attachment and spreading. This nonlinear dependence may be explained by nonhomogeneous RGD surface distribution at the nanometre scale, conditioned by the stochastic nature of the hydrolysis process. Atomic force microscopy analysis of the gradient surface showed an evolution of surface morphology compatible with this hypothesis.
Continuous Bone Morphogenetic Protein-2 Gradients for Concentration Effect Studies on C2C12 Osteogenic Fate Nanomedicine : Nanotechnology, Biology, and Medicine. | Pubmed ID: 23313904 Cells can respond to small changes in a varying concentration of exogenous signaling molecules. Here we propose the use of continuous surface chemical gradients for the in-depth study of dose-dependent effects on cells. A continuous surface gradient of bone morphogenetic protein-2 (BMP-2) is presented. The gradient covers a narrow range of surface densities (from 1.4 to 2.3 pmol/cm(2)) with a shallow slope (0.9 pmol/cm(3)). These characteristics represent a quasi-homogeneous surface concentration at the cell scale, which is crucial for cell screening studies. Cell fate evaluation at early stages of osteogenesis in C2C12 cells, indicates the potential of continuous gradients for in vitro screening applications.