Articles by Anne Fiedler in JoVE
Geração de humanos CD40-activated células B Tanja M. Liebig1, Anne Fiedler1, Shahram Zoghi1, Alexander Shimabukuro-Vornhagen1, Michael S. von Bergwelt-Baildon1 1Laboratory for Tumor and Transplantation Immunology and Stem Cell Transplantation Program, University Hospital of Cologne, Department I of Internal Medicine Neste vídeo, apresentamos a
Modelo murino de CD40-ativação de células B Tanja M. Liebig1, Anne Fiedler1, Nela Klein-Gonzalez1, Alexander Shimabukuro-Vornhagen1, Michael von Bergwelt-Baildon1 1Laboratory for Tumor and Transplantation Immunology, Department I of Internal Medicine, University Hospital of Cologne Neste vídeo, demonstramos o procedimento de ativação CD40-e expansão de células B murino de esplenócitos de camundongos C57BL / 6, que pode ser usado como um modelo de células apresentadoras de antígenos (APC) para estudar a indução de imunidade.
Other articles by Anne Fiedler on PubMed
Chemical Conjugation of Linear and Cyclic RGD Moieties to a Recombinant Elastin-mimetic Polypeptide--a Versatile Approach Towards Bioactive Protein Hydrogels Macromolecular Bioscience. Jun, 2008 | Pubmed ID: 18350537 An elastin-mimetic polypeptide, (EMM)(7), with the amino-acid sequence GRDPSS [VPGVG VPGKG VPGVG VPGVG VPGEG VPGIG](7) was used for chemical conjugation of various integrin ligands (RGD peptides) to prepare bioactive hydrogels. The chemical approach involved (1) chemical protection of lysine residues with Fmoc or Boc groups, (2) chemical ligation of a protected linear or cyclic RGD ligand, with or without a hexanoic-acid spacer to the glutamic acid residue, (3) deprotection of the lysine functionalities and the RGD moieties and (4) cross-linking to form a bioactive hydrogel. (1)H NMR spectroscopy was used to quantify the multiple steps in the reaction. The chemical protection was found to be between 65 and 93% for Fmoc and Boc, respectively. The ligands studied included linear RGD cell-binding [H-FGRGDS-OH (1-l-RGD), H-Ahx--FGRGDS-OH (2-Ahx-FGRGDS) and a cyclic -H(2)N-(CH(2))(6)COHN-cyclo(-RGDfK-) (H-Ahx-c(-RGDfK-)) peptide also with a hexanoic-acid spacer. Cell adhesion with mouse osteoblast cells was dependent on the ligand type, ligand density and the use of a spacer.