Articles by Ariane C. C. van Spreeuwel in JoVE
Os tecidos músculo esquelético de engenharia a partir de células progenitoras mioblastos murinos e Aplicação da estimulação elétrica Daisy W. J. van der Schaft1, Ariane C. C. van Spreeuwel1, Kristel J. M. Boonen1, Marloes L. P. Langelaan1, Carlijn V. C. Bouten1, Frank P. T. Baaijens1 1Department of Biomedical Engineering, Soft Tissue Biomechanics and Engineering, Eindhoven University of Technology, The Netherlands Tecido muscular de engenharia tem um grande potencial na medicina regenerativa, como modelo de doença e também como uma fonte alternativa para a carne. Aqui descreve-se a engenharia de uma construção de músculo, neste caso, a partir de células progenitoras, mioblastos de rato e a estimulação por pulsos eléctricos.
Other articles by Ariane C. C. van Spreeuwel on PubMed
Mechanoregulation of Vascularization in Aligned Tissue-engineered Muscle: a Role for Vascular Endothelial Growth Factor Tissue Engineering. Part A. Nov, 2011 | Pubmed ID: 21702712 Skeletal muscle tissue engineering has major promise for regenerative treatment of patients suffering from muscle loss due to, for example, traumatic injury, but faces considerable challenges to progress toward clinical application. In the present study the creation of an aligned prevascularized muscle tissue was addressed. We hypothesized that an aligned vascularized three-dimensional (3D) muscle tissue can be induced in vitro by merely using uniaxial stress. The present study showed that not only do endothelial cells and muscle cells independently align in the direction of uniaxial stress in a hydrogel-based 3D culture system, but also, more importantly, the endothelial cells in the co-cultured 3D constructs organized into vascular structures. Strikingly, in these cultures no additional growth factors were needed to induce vascular formation of the endothelial cells. Vascular endothelial growth factor (VEGF) production by the muscle cells was stimulated by the uniaxial stress that develops in the tissue when constrained in one direction. This stress accompanied by VEGF production appeared to play a key role in the organization of the endothelial cells into vessel-like structures.