In JoVE (1)
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Articles by Arivarasu N. Anabazhagan in JoVE
Methods to Study Epithelial Transport Protein Function and Expression in Native Intestine and Caco-2 Cells Grown in 3D Arivarasu N. Anabazhagan*1, Ishita Chatterjee*1, Shubha Priyamvada1, Anoop Kumar1, Sangeeta Tyagi1, Seema Saksena1,2, Waddah A. Alrefai1,2, Pradeep K. Dudeja1,2, Ravinder K. Gill1 1Department of Medicine, University of Illinois at Chicago, 2Department of Research, Jesse Brown VA Medical Center We describe simple methods to study the regulation of intestinal serotonin transporter (SERT) function and expression using an in vitro cell culture model of Caco-2 cells grown in 3D and an ex vivo model of mouse intestines. These methods are applicable to the study of other epithelial transporters.