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Articles by Ashley H. Ramsawhook in JoVE
Immunostaining for DNA Modifications: Computational Analysis of Confocal Images Ashley H. Ramsawhook*1, Lara C. Lewis*1, Maria Eleftheriou1, Abdulkadir Abakir1, Paulina Durczak1, Robert Markus2, Seema Rajani2, Nicholas R.F. Hannan1, Beth Coyle3, Alexey Ruzov1 1Division of Cancer and Stem Cells, School of Medicine, Centre for Biomolecular Sciences, University of Nottingham, 2School of Life Sciences Imaging (SLIM), School of Life Sciences, University of Nottingham, 3Children's Brain Tumour Research Centre, School of Medicine, QMC, University of Nottingham Newly discovered oxidized forms of 5-methylcytosine (oxi-mCs), 5-hydroxymethylcytosine (5hmC), 5-formylcytosine (5fC) and 5-carboxylcytosine (5caC) may represent distinct DNA modifications with unique functional roles. Here a semi-quantitative workflow for visualization of oxi-mCs' spatial distribution, signal intensity profiling and colocalization is described.