Articles by Assaf A Gilad in JoVE
Synthesis of Multi-walled Carbon Nanotubes Modified with Silver Nanoparticles and Evaluation of Their Antibacterial Activities and Cytotoxic Properties Youngmin Seo*1, Chanhwi Park*2, Jaewoo Son2, Kyungwoo Lee2, Jangsun Hwang2, Yeonho Jo2, Dohyun Lee2, Muhammad Saad Khan2, Sachin Ganpat Chavan2, Yonghyun Choi2, Dasom Kim2, Assaf A Gilad3, Jonghoon Choi2 1Center for Biomaterials, Biomedical Research Institute, Korea Institute of Science and Technology, 2School of Integrative Engineering, Chung-Ang University, 3Division of Synthetic Biology and Regenerative Medicine, Institute for Quantitative Health Science and Engineering, Michigan State University In this study, antimicrobial nanomaterials were synthesized by acidic oxidation of multiwalled carbon nanotubes and subsequent reductive deposition of silver nanoparticles. Antimicrobial activity and cytotoxicity tests were performed with the as-prepared nanomaterials.
Other articles by Assaf A Gilad on PubMed
Non-invasive Detection of Adeno-associated Viral Gene Transfer Using a Genetically Encoded CEST-MRI Reporter Gene in the Murine Heart Scientific Reports. | Pubmed ID: 29545551 Research into gene therapy for heart failure has gained renewed interest as a result of improved safety and availability of adeno-associated viral vectors (AAV). While magnetic resonance imaging (MRI) is standard for functional assessment of gene therapy outcomes, quantitation of gene transfer/expression relies upon tissue biopsy, fluorescence or nuclear imaging. Imaging of gene expression through the use of genetically encoded chemical exchange saturation transfer (CEST)-MRI reporter genes could be combined with clinical cardiac MRI methods to comprehensively probe therapeutic gene expression and subsequent outcomes. The CEST-MRI reporter gene Lysine Rich Protein (LRP) was cloned into an AAV9 vector and either administered systemically via tail vein injection or directly injected into the left ventricular free wall of mice. Longitudinal in vivo CEST-MRI performed at days 15 and 45 after direct injection or at 1, 60 and 90 days after systemic injection revealed robust CEST contrast in myocardium that was later confirmed to express LRP by immunostaining. Ventricular structure and function were not impacted by expression of LRP in either study arm. The ability to quantify and link therapeutic gene expression to functional outcomes can provide rich data for further development of gene therapy for heart failure.