Articles by Barbara E. Taylor in JoVE
Caenorhabditis Sieve: A Low-tech Instrument and Methodology for Sorting Small Multicellular Organisms Skyler Hunter*1,2, Malabika Maulik*3, Courtney Scerbak4, Elena Vayndorf2, Barbara E. Taylor5 1Department of Biology and Wildlife, University of Alaska Fairbanks, 2Institute of Arctic Biology, University of Alaska Fairbanks, 3Department of Chemistry and Biochemistry, University of Alaska Fairbanks, 4Departments of Biology and Chemistry, Earlham College, 5Department of Biological Sciences, College of Natural Science and Mathematics, California State University Long Beach The current protocol includes a methodology for the sorting and cleaning of age-matched populations of Caenorhabditis elegans. It uses a simple, inexpensive, and efficient custom-made tool to obtain a large experimental population of nematodes for research.
Other articles by Barbara E. Taylor on PubMed
The Rostral Medulla of Bullfrog Tadpoles Contains Critical Lung Rhythmogenic and Chemosensitive Regions Across Metamorphosis Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology. | Pubmed ID: 29890210 The development of amphibian breathing provides insight into vertebrate respiratory control mechanisms. Neural oscillators in the rostral and caudal medulla drive ventilation in amphibians, and previous reports describe ventilatory oscillators and CO sensitive regions arise during different stages of amphibian metamorphosis. However, inconsistent findings have been enigmatic, and make comparisons to potential mammalian counterparts challenging. In the current study we assessed amphibian central CO responsiveness and respiratory rhythm generation during two different developmental stages. Whole-nerve recordings of respiratory burst activity in cranial and spinal nerves were made from intact or transected brainstems isolated from tadpoles during early or late stages of metamorphosis. Brainstems were transected at the level of the trigeminal nerve, removing rostral structures including the nucleus isthmi, midbrain, and locus coeruleus, or transected at the level of the glossopharyngeal nerve, removing the putative buccal oscillator and caudal medulla. Removal of caudal structures stimulated the frequency of lung ventilatory bursts and revealed a hypercapnic response in normally unresponsive preparations derived from early stage tadpoles. In preparations derived from late stage tadpoles, removal of rostral or caudal structures reduced lung burst frequency, while CO responsiveness was retained. Our results illustrate that structures within the rostral medulla are capable of sensing CO throughout metamorphic development. Similarly, the region controlling lung ventilation appears to be contained in the rostral medulla throughout metamorphosis. This work offers insight into the consistency of rhythmic respiratory and chemosensitive capacities during metamorphosis.