In JoVE (1)
Other Publications (1)
Articles by Bauer L. LeSavage in JoVE
Production of Elastin-like Protein Hydrogels for Encapsulation and Immunostaining of Cells in 3D Bauer L. LeSavage*1, Nicholas A. Suhar*2, Christopher M. Madl1, Sarah C. Heilshorn2 1Department of Bioengineering, Stanford University, 2Department of Materials Science and Engineering, Stanford University Recombinant protein-engineered hydrogels are advantageous for 3D cell culture as they allow for complete tunability of the polymer backbone and therefore, the cell microenvironment. Here, we describe the process of recombinant elastin-like protein purification and its application in 3D hydrogel cell encapsulation.
Other articles by Bauer L. LeSavage on PubMed
Maintenance of Neural Progenitor Cell Stemness in 3D Hydrogels Requires Matrix Remodelling Nature Materials. | Pubmed ID: 29115291 Neural progenitor cell (NPC) culture within three-dimensional (3D) hydrogels is an attractive strategy for expanding a therapeutically relevant number of stem cells. However, relatively little is known about how 3D material properties such as stiffness and degradability affect the maintenance of NPC stemness in the absence of differentiation factors. Over a physiologically relevant range of stiffness from ∼0.5 to 50 kPa, stemness maintenance did not correlate with initial hydrogel stiffness. In contrast, hydrogel degradation was both correlated with, and necessary for, maintenance of NPC stemness. This requirement for degradation was independent of cytoskeletal tension generation and presentation of engineered adhesive ligands, instead relying on matrix remodelling to facilitate cadherin-mediated cell-cell contact and promote β-catenin signalling. In two additional hydrogel systems, permitting NPC-mediated matrix remodelling proved to be a generalizable strategy for stemness maintenance in 3D. Our findings have identified matrix remodelling, in the absence of cytoskeletal tension generation, as a previously unknown strategy to maintain stemness in 3D.