Articles by Beatriz Martí-Prado in JoVE
Stable and Efficient Genetic Modification of Cells in the Adult Mouse V-SVZ for the Analysis of Neural Stem Cell Autonomous and Non-autonomous Effects Eva Porlan1, Beatriz Martí-Prado2,3, Antonella Consiglio4,5, Isabel Fariñas2,3 1Cell Division and Cancer Group, Spanish National Cancer Research Centre (CNIO), 2Centro de Investigaciones Biomédicas en Red sobre Enfermedades Neurodegenerativas (CIBERNED), 3Departmento de Biologìa Celular, Universidad de Valencia, 4Institut de Biomedicina de la Universitat de Barcelona (IBUB), 5Department of Molecular and Translational Medicine, Fibroblast Reprogramming Unit, University of Brescia Here we describe a procedure based on the use of lentiviral particles for the long-term genetic modification of neural stem cells and/or their adjacent ependymal cells in the adult ventricular-subventricular neurogenic niche which allows the separate analysis of cell autonomous and non-autonomous, niche-dependent effects on neural stem cells.
Other articles by Beatriz Martí-Prado on PubMed
MT5-MMP Regulates Adult Neural Stem Cell Functional Quiescence Through the Cleavage of N-cadherin Nature Cell Biology. Jul, 2014 | Pubmed ID: 24952463 The identification of mechanisms that maintain stem cell niche architecture and homeostasis is fundamental to our understanding of tissue renewal and repair. Cell adhesion is a well-characterized mechanism for developmental morphogenetic processes, but its contribution to the dynamic regulation of adult mammalian stem cell niches is still poorly defined. We show that N-cadherin-mediated anchorage of neural stem cells (NSCs) to ependymocytes in the adult murine subependymal zone modulates their quiescence. We further identify MT5-MMP as a membrane-type metalloproteinase responsible for the shedding of the N-cadherin ectodomain in this niche. MT5-MMP is co-expressed with N-cadherin in adult NSCs and ependymocytes and, whereas MT5-MMP-mediated cleavage of N-cadherin is dispensable for the regulation of NSC generation and identity, it is required for proper activation of NSCs under physiological and regenerative conditions. Our results indicate that the proliferative status of stem cells can be dynamically modulated by regulated cleavage of cell adhesion molecules.