Other Publications (1)
Articles by Benem Davids in JoVE
Measurement of In Vitro Integration Activity of HIV-1 Preintegration Complexes Muthukumar Balasubramaniam1,2, Benem Davids1,2, Amma B. Addai1,2, Jui Pandhare1,3,4, Chandravanu Dash1,2,5 1Center for AIDS Health Disparities Research, Meharry Medical College, 2Department of Biochemistry and Cancer Biology, Meharry Medical College, 3School of Graduate Studies and Research, Meharry Medical College, 4Department of Microbiology and Immunology, Meharry Medical College, 5Tennessee Center for AIDS Research (TN-CFAR), Meharry Medical College This report describes an in vitro assay for measuring the human immunodeficiency virus type 1 (HIV-1) preintegration complex (PIC)-associated integration activity in the cytoplasmic extracts of acutely infected cells. The integration of PIC-associated HIV-1 DNA into heterologous target DNA is quantified by using a nested real-time polymerase chain reaction strategy.
Other articles by Benem Davids on PubMed
Bilateral Gene Interaction Hierarchy Analysis of the Cell Death Gene Response Emphasizes the Significance of Cell Cycle Genes Following Unilateral Traumatic Brain Injury BMC Genomics. Feb, 2016 | Pubmed ID: 26912237 Delayed or secondary cell death that is caused by a cascade of cellular and molecular processes initiated by traumatic brain injury (TBI) may be reduced or prevented if an effective neuroprotective strategy is employed. Microarray and subsequent bioinformatic analyses were used to determine which genes, pathways and networks were significantly altered 24 h after unilateral TBI in the rat. Ipsilateral hemi-brain, the corresponding contralateral hemi-brain, and naïve (control) brain tissue were used for microarray analysis.